Team:Warsaw/Calendar-Main/21 May 2008

From 2008.igem.org

(Difference between revisions)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
-
<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3>
+
<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7<br>
-
<h4>Michał K.:</h4>
+
Michał K.</h3>
<p><ol>
<p><ol>
<li> Inoculation separate transformant colonies for transcription fusion (liquid LB + tetracycline).</li>
<li> Inoculation separate transformant colonies for transcription fusion (liquid LB + tetracycline).</li>
Line 10: Line 10:
</ol></p>
</ol></p>
 +
<h3> Results of rifampicin test<br>
 +
Michał L., Ewa, Marcin</h3>
-
<h4>Michał L., Ewa, Marcin:</h4>
 
-
<h3> Results of rifampicin test</h3>
 
<table id="result">
<table id="result">

Revision as of 14:35, 9 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7
Michał K.

  1. Inoculation separate transformant colonies for transcription fusion (liquid LB + tetracycline).
  2. Inoculation separate transformant colonies for translation fusion (liquid LB + tetracycline).

Results of rifampicin test
Michał L., Ewa, Marcin

StrainInducer / repressorNumber of colonies on rifampicin
Top10none12
Top1010mM IPTG17
Top10 pMPM-AIDnone13
Top10 pMPM-AID1% Glucose18
Top10 pMPM-AID0.1% Arabinose54
Top10 pTRC-AIDnone14
Top10 pTRC-AID1% Glucose17
Top10 pTRC-AID0.1% Arabinose53

Conclusion: AID seems to work!

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/21_May_2008"