Team:Warsaw/Calendar-Main/21 October 2008
From 2008.igem.org
(Difference between revisions)
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103019>alpha_linker under PT7 (BBa_K103019)</a>).</li> | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103019>alpha_linker under PT7 (BBa_K103019)</a>).</li> | ||
- | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol> | + | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/21_October_2008#fig1">Fig. 1</a>.</li></ol> |
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/1/11/Traw2_19_10_2008.jpg"></a> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/1/11/Traw2_19_10_2008.jpg"></a> | ||
- | <var><b>Fig. | + | <var><b>Fig. 1.</b> EcoRI/PSTI digest of pT7_alpha_link </var> |
- | + | 1. Marker<br> | |
+ | 2-3. EcoRI/PstI digests of pT7_alpha_link<br> </var> | ||
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day - <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> +<a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a>.</li> | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day - <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> +<a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a>.</li> | ||
- | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.</li> | + | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/21_October_2008#fig2">Fig. 2</a>.</li> |
</ol> | </ol> | ||
- | <a name=" | + | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/a/ac/Traw4_16_10_2008.jpg"></a> |
- | <var><b>Fig. | + | <var><b>Fig. 2.</b> EcoRI/PstI digests of BBa_K103002 <br> |
+ | 1. Marker <br> | ||
+ | 2-3. EcoRI/PstI digests of BBa_K103002 <br></var> | ||
+ | |||
Revision as of 13:31, 28 October 2008
Preparation of OmpA-linker-omega-linker (BBa_K103016)PiotrInoculation of few more colonies from plate with ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 16 October) to LB with kanamycin. Preparation of alpha_linker under PT7 (BBa_K103019)Michał K.
2-3. EcoRI/PstI digests of pT7_alpha_link Preparation of AID under pBAD/araC (BBa_K103002)Michał K.
1. Marker 2-3. EcoRI/PstI digests of BBa_K103002 Preparation of OmpA_linker_alpha_linker (BBa_K103014)Michał K.Inoculation of colonies from plate with ligation (pSB2K3 + OmpA_linker_alpha_linker (BBa_K103014)) to LB with kanamycin. |