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Team:Warsaw/Calendar-Main/22 August 2008
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<h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid<br>Antoni</h3> | <h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid<br>Antoni</h3> | ||
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| - | <li> Isolation of plasmids from cultures inocluated on previous day (pGeneart+A and pET15b+OmpA-alfa). </li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/21_August_2008">previous day</a> (pGeneart+A and pET15b+OmpA-alfa). </li> |
<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a> </li> </li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a> </li> </li> | ||
<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li> | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li> | ||
Revision as of 21:23, 8 October 2008
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Cloning of protein A DNA to pET15b+Omp-alfa plasmid
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