Team:Warsaw/Calendar-Main/22 May 2008

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Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br>
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br>
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Annealing temperature : 60&dgr;C <br>
+
Annealing temperature : 60&deg;C <br>
Annealing time: 4 minutes <br>
Annealing time: 4 minutes <br>

Revision as of 21:59, 30 September 2008

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Piotr:

1. Design of primers for sequencing something we want from pMPM-T5

Primers:

pMPMT5seqF pMPMT5seqR AIDseqP

Michał K.: 1. PCR - transcription fusion: AID + T7 RNA-polymerase</p>

Primers:

AIDlNrH and T7pXbSal


Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing temperature : 60°C
Annealing time: 4 minutes

35 cycles