Team:Warsaw/Calendar-Main/22 May 2008

From 2008.igem.org

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Michał K.:<br>
Michał K.:<br>
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1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - transcription fusion: AID + T7 RNA-polymerase
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1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase
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<p>Primers: </p>
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<p><html>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html></p>
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Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br>
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Annealing temperature : 60&deg;C <br>
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Annealing time: 4 minutes <br>
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20 cycles <br>
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2. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase
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<p>Primers: </p>
<p>Primers: </p>

Revision as of 22:51, 30 September 2008

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Piotr:

1. Design of primers for sequencing something we want from pMPM-T5

Primers:

pMPMT5seqF pMPMT5seqR AIDseqP

Michał K.:
1. PCR - translation fusion: AID + T7 RNA-polymerase

Primers:

AIDlNrH and T7pXbSal</p>


Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing temperature : 60°C
Annealing time: 4 minutes

20 cycles
3. Gel electrophoresis and isolation of proper bands (3300 bp).

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