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Team:Warsaw/Calendar-Main/22 May 2008
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li> | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li> | ||
| - | <li> Gel electrophoresis (no proper colonies found).</li></html> | + | <li> Gel electrophoresis (no proper colonies found).</li> |
| + | <img src="https://static.igem.org/mediawiki/2008/8/87/Aid_fusion_digests_WAW.jpg"width=300/> | ||
| + | <h3>1-DNA ladder; 2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); 4-pMPM-T5-AID digested with EcoRI; 5-hypothetical pMPM with translation fusion digested with BamHI; 6-pMPM-T5-AID digested with BamHI.</h3> | ||
| + | </html> | ||
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Revision as of 08:08, 6 October 2008
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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7Piotr:
Primers: Michał K.:
1-DNA ladder; 2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); 4-pMPM-T5-AID digested with EcoRI; 5-hypothetical pMPM with translation fusion digested with BamHI; 6-pMPM-T5-AID digested with BamHI. |
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