Team:Warsaw/Calendar-Main/22 May 2008

From 2008.igem.org

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<h4>Piotr:</h4>
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Design of primers for sequencing something we want from pMPM-T5<br>
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Piotr:
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Primers:<br>
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1. Design of primers for sequencing something we want from pMPM-T5
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<ul>
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<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pMPMT5seqF">pMPMT5seqF</a></li>
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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pMPMT5seqR">pMPMT5seqR</a></li>
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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a></li></ul>
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Primers:
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<h4>Michał K.:</h4>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase<br>
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Primers: <br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pMPMT5seqF">pMPMT5seqF</a>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pMPMT5seqR">pMPMT5seqR</a>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a>
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Michał K.:<br>
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1.  <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase
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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a></li>  
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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></li>
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<p>Primers: </p>
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<p><html>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html>
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Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion <br>
Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion <br>
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Annealing temperature : 73&deg;C <br>
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Elongation temperature : 73&deg;C <br>
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Annealing time: 4 minutes <br>
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Elongation time: 4 minutes <br>
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20 cycles <br>
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2. Gel electrophoresis and isolation of proper band (3300 bp).<br>
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20 cycles <br></li>
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<li> Gel electrophoresis and isolation of proper band (3300 bp).</li></ol></p>
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Revision as of 18:45, 1 October 2008

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Piotr:

Design of primers for sequencing something we want from pMPM-T5
Primers:

Michał K.:

  1. PCR - translation fusion: AID + T7 RNA-polymerase
    Primers:
    Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
    Elongation temperature : 73°C
    Elongation time: 4 minutes
    20 cycles
  2. Gel electrophoresis and isolation of proper band (3300 bp).

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