Preparation of pMPMT5-AID+AIDT7 construct

PCR - translation fusion: AID + T7 RNA-polimerase
Primers: AIDlNrH and T7pXbSal
Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion
Elongation temperature: 55°C
Elongation time: 4 minutes
Number of cycles: 20 and 25 (2 repeats)

Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles)

PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.