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Team:Warsaw/Calendar-Main/25 August 2008
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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | <h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | ||
| - | <p> <ol><li>Purification | + | <p> <ol><li>Purification OmpA_alpha of antibiotic resistance contamination:<br> |
Inoculation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> careing pACTC177+OmpA_alpha to liquin LB with kanamycin and with kanamycin and ampicylin.</li> | Inoculation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> careing pACTC177+OmpA_alpha to liquin LB with kanamycin and with kanamycin and ampicylin.</li> | ||
<li>Inoculation of TOP10 carrying OmpA_A_alpha and OmpA-alpha to LB with inductor (0.25 mM IPTG) and kanamycin.</p> | <li>Inoculation of TOP10 carrying OmpA_A_alpha and OmpA-alpha to LB with inductor (0.25 mM IPTG) and kanamycin.</p> | ||
Revision as of 18:07, 24 October 2008
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Tests for ampicillin resistance given by protein added to mediumPiotr
Cloning of protein A DNA to pET15b+Omp-alpha plasmidAntoni
Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.Eight colonies from each plate (pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin.
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