Team:Warsaw/Calendar-Main/25 September 2008

From 2008.igem.org

Revision as of 20:11, 22 October 2008 by MKrzyszton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Mutagenesis of protein A

Paweł

Treatment of mutageneses as on 23rd September.

Preparation of alpha_A construct

Antoni

  1. PCR on alpha_linker and linker_A with AlphaL+SacI and AP+NotI primers and 10% DMSO (30 cycles, elongation 60 s, annealing temperature 72°C).
  2. Gel electrophoresis of PCR products and gel-out of proper band (1019 bp).
  3. Measurment of concentration of both isolated products.

Preparation of BioBricks

Piotr

  1. Colony PCR with AL_BNXNE and APSacSpe primers on colonies from plates with transformations RFP(??????????) + deltaA. No products visible.
  2. Inoculation of some colonies from plate to LB with ampicillin