"
Team:Warsaw/Calendar-Main/27 May 2008
From 2008.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
<html> | <html> | ||
| - | < | + | <h4>Michał K.:</h4> |
| + | <p><ol> | ||
| + | <li> <a href:"https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with transcription fusion.</li> | ||
| - | < | + | <li> <a href:"https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translation fusion.</li> |
| - | < | + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).</li> |
| - | < | + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li> |
| - | < | + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).</li> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | <li> Choice of proper clones and preparation for sequencing.</li> | ||
| + | </ol></p> | ||
<h3>Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br> | <h3>Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br> | ||
| - | + | Digest was conducted with BamHI</h3> | |
</html> | </html> | ||
<center>https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg</center> | <center>https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg</center> | ||
Revision as of 18:49, 1 October 2008
 |
|
||||||||
 |
|
||||||||
Michał K.:
Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since.
|
|
