Team:Warsaw/Calendar-Main/29 July 2008

From 2008.igem.org

(Difference between revisions)
Line 20: Line 20:
<tr><th rowspan="2">ampicillin concentration (mikrogramy na mililitr):</th><th colspan="6">IPTG concentration (milimole na mililitr):</td></tr>
<tr><th rowspan="2">ampicillin concentration (mikrogramy na mililitr):</th><th colspan="6">IPTG concentration (milimole na mililitr):</td></tr>
<tr><th>0</th><th>0,1</th><th>0,25</th><th>0,5</th><th>0,75</th><th>1</th></tr>
<tr><th>0</th><th>0,1</th><th>0,25</th><th>0,5</th><th>0,75</th><th>1</th></tr>
-
<tr><th>25</th><td class="dead">1,558</td><td class="live">1,469</td><td class="dead">1,587</td><td class="live">1,49</td><td class="dead">1,566</td><td class="live">1,311</td></tr>
+
<tr><th>25</th><td class="live">1,558</td><td class="live">1,469</td><td class="live">1,587</td><td class="live">1,49</td><td class="live">1,566</td><td class="live">1,311</td></tr>
-
<tr><th>50</th><td class="live">1,425</td><td class="dead">1,435</td><td class="live">1,524</td><td class="dead">1,055</td><td class="live">0,920</td><td class="dead">0,935</td></tr>
+
<tr><th>50</th><td class="live">1,425</td><td class="live">1,435</td><td class="live">1,524</td><td class="live">1,055</td><td class="live">0,920</td><td class="live">0,935</td></tr>
-
<tr><th>75</th><td class="dead">1.09</td><td class="live">0,989</td><td class="dead">1.447</td>
+
<tr><th>75</th><td class="live">1.09</td><td class="live">0,989</td><td class="live">1.447</td><td class="live">0.971</td><td class="live">0.951</td><td class="live">0.992</td>
</tr>
</tr>
<tfoot>
<tfoot>

Revision as of 07:55, 9 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



1. Separete transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.
2. Inoculation of pACYC177+OmpA_alpha and pACYC177+OmpA_omega - liquid LB with kanamycin.

Sprawdzanie czy omp_omega_A_alfa daje oporność na AP Piotr Zaszczepienie omp_omega_A_alfa z rużnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75; 1 do takich samych stężeń IPTG, ale z rużnymi stężeniami AP (25;50;75;100) w stosunku 1:50 wieczorem mieżenie OD :

Table 2. Results of testing various hunter/prey combinations
(ampicillin concentration 50-75 ug/ml).

ampicillin concentration (mikrogramy na mililitr):IPTG concentration (milimole na mililitr):
00,10,250,50,751
251,5581,4691,5871,491,5661,311
501,4251,4351,5241,0550,9200,935
751.090,9891.4470.9710.9510.992
Zaszczepienie omp_omega_A_alfa na rużnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75 (do powtużenia ) Sprawdzenie ekspresji omp_omega_A_alfa i omp_A_alfa Piotr Zwirowanie, zawieszenie, dodanie buforu lizującego (skład), zagotowanie, nałożenie na żel poliakryl, transfer na nitrocelulozę, blokowanie, przeciwciała anty-A, płukanie, przeciwciała anty-królik, wywołanie BCIP i NBT (omp_omega_A_alfa, z i bez indukcji, omp_A_alfa) (Zdjęcie żelu)