Team:Warsaw/Calendar-Main/29 May 2008

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Rifampicin test #3

Michał L., Ewa, Marcin, Piotr

  1. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB.
  2. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.
  3. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB.
  4. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1 mM IPTG.
  5. Cultures were incubated for 5 hours.
  6. 1 ml (2x) from p.1, p.2, p.3, p.4 were plated on LB + rifampicin.
  7. OD measurement of above cultures.

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