Team:Warsaw/Calendar-Main/29 September 2008
From 2008.igem.org
(Difference between revisions)
Line 10: | Line 10: | ||
- | <h3>Preparation of pACYC177 + | + | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
Line 16: | Line 16: | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>pACYC177+OmpA_omega_ΔA_alpha</a> plasmid using | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>pACYC177+OmpA_omega_ΔA_alpha</a> plasmid using | ||
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmLNXNEB">OmLNXNEB</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinPBSNP">LinPBSNP</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmLNXNEB">OmLNXNEB</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinPBSNP">LinPBSNP</a> | ||
- | primers (annealing temperature 58 °C; elongation length 90s) to obtain | + | primers (annealing temperature 58 °C; elongation length 90s) to obtain <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> fragment. </li> |
- | <li> Gel electrophoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands ( | + | <li> Gel electrophoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (OmpA-linker-omega-linker - 900 bp).</li> |
Revision as of 00:10, 27 October 2008
MutD5 testingPiotrSequencing results: there weren't any mutations in plasmids isolated from MutD5 - maybe this strain is too weak as a mutator. Preparation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of BioBricksMichał K.
Preparation of vectors for BiobricksPiotrInoculation of bacteria received from iGEM HQs, carrying pSB2K3 and BBa_I739204 (pACYC177 converted into BioBrick vector) plasmids. Fig. 1. PCR on pLac_OmpA_omega_1. Marker 2. PCR product
|