Team:Warsaw/Calendar-Main/2 June 2008

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<h4>PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase</h4><p>
<h4>PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase</h4><p>
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Primers: AIDlNrH and T7pXbSal<br>
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Primers:  
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br></html>
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br>
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br>

Revision as of 13:52, 26 September 2008

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Piotr: Transformation of E.coli TOP10 with pZC320.

Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase

Primers: AIDlNrH and T7pXbSal

Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)

Annealing temperature: 55°C

Time: 4 minutes

Number of cycles: 20