Team:Warsaw/Calendar-Main/2 June 2008

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1.<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with pZC320.</p>
1.<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with pZC320.</p>
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<p>2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</p>
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<p>2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</html></p>
Michał K.: <br>
Michał K.: <br>

Revision as of 13:35, 1 October 2008

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Piotr:
1.Transformation of E.coli TOP10 with pZC320.

2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</p>

Michał K.:

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase

Primers:

<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
</html>

Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)

Annealing temperature: 55°C

Time: 4 minutes

Number of cycles: 20