Team:Warsaw/Calendar-Main/2 June 2008

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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</li>
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<li> Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</li></ol></p>
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<li> Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</li></ol><br></p>
<h3>Preparation of pMPMT5-AID+AIDT7 construct</h3>
<h3>Preparation of pMPMT5-AID+AIDT7 construct</h3>
<h4>Michał K.:</h4>
<h4>Michał K.:</h4>

Revision as of 17:45, 5 October 2008

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Michał L., Ewa, Marcin:

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.

Preparation of pMPMT5-AID+AIDT7 construct

Michał K.:

  1. PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase.

    2. Primers:
    AIDlNrH and T7pXbSal
    Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
    Elongation temperature: 55°C
    Time: 4 minutes
    Number of cycles: 20


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