Team:Warsaw/Calendar-Main/2 June 2008

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Revision as of 09:13, 6 October 2008

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Michał L., Ewa, Marcin:

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.

Preparation of pMPMT5-AID+AIDT7 construct

Michał K.:

  1. PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase.

    2. Primers:
    AIDlNrH and T7pXbSal
    Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
    Elongation temperature: 55°C
    Time: 4 minutes
    Number of cycles: 20 and 25


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