Team:Warsaw/Calendar-Main/2 June 2008

From 2008.igem.org

(Difference between revisions)
Line 3: Line 3:
<html>
<html>
 +
 +
<h3> Results of rifampicin test and OD measurement</h3>
 +
<h4>Piotr</h4>
 +
<table id="result">
 +
<tr><th>Strain</th><th>Inducer</th><th>Number of colonies on rifampicin</th></tr><th>OD</th></tr>
 +
<tr><th>Top10</th><td>none</td><td>100</td></tr><td>2.66</td></tr>
 +
<tr><th>Top10 pMPM-AID</th><td>none</td><td>15</td></tr><td>2.5</td></tr>
 +
<tr><th>Top10 pMPM-AID</th><td>0.1% Arabinose</td><td>42</td></tr><td>2.17</td></tr>
 +
<tr><th>Top10 pTRC-AID</th><td>none</td><td>12</td></tr><td>2.61</td></tr>
 +
<tr><th>Top10 pTRC-AID</th><td>0.5 mM IPTG</td><td>35</td></tr><td>1.98</td></tr>
 +
</table>
 +
<br/>
 +
<p>Conclusion: AID seems to work, induction of AID reduces the growth rate</p>
 +
<h3>Preparation of pMPMT5-AID+AIDT7 construct</h3>
<h3>Preparation of pMPMT5-AID+AIDT7 construct</h3>

Revision as of 12:13, 19 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Results of rifampicin test and OD measurement

Piotr

StrainInducerNumber of colonies on rifampicin
OD
Top10none100
2.66
Top10 pMPM-AIDnone15
2.5
Top10 pMPM-AID0.1% Arabinose42
2.17
Top10 pTRC-AIDnone12
2.61
Top10 pTRC-AID0.5 mM IPTG35
1.98

Conclusion: AID seems to work, induction of AID reduces the growth rate

Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

PCR for AID transcriptional fusion with translational fusion AID+T7 RNA-polymerase.
Primers: AIDlNrH and T7pXbSal
Template DNA: translational fusion AID+T7 RNA-polymerase (pMPMT5+AID-T7)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20 and 25

Blue/white and rifampicin test

Michał L., Ewa, Marcin

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin (30 μg/mL) + X-gal + IPTG.