Team:Warsaw/Calendar-Main/30 July 2008

From 2008.igem.org

(Difference between revisions)

Revision as of 13:32, 9 October 2008


	Attribution analyzer / Interactive list of topics

1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids (pACYC177+omega_deltaA) with HindIII and SacI (7 from 8 colonies confirmed).

Piotr
Checking if OmpA_omega_A_alpha gives ampicillin resistance Inoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 into the same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100) in relation 1:50 Bacterial growth (OD) measurement in the evening:

ampicillin concentration (mikrogramy na mililitr):	IPTG concentration (milimole na mililitr):
ampicillin concentration (mikrogramy na mililitr):	0	0,1	0,25	0,5	0,75	1
25	1,054	1,154	1,051	0,99	1,096	0,896
50	0,94	0,891	1,123	0,847	0,924	0,81
75	0.5	0,63	0.743	0.782	0.631	0.64
100	0.02	0,396	0.563	0.678	0.602	0.611

Checking if degradation of fusion with OmpA is a result of the activity of lon and iompt proteases. (present in top10) Transformation omega_A_alpha and A_alpha into Rosetta.

@@ Line 7: / Line 7: @@
 . Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids (pACYC177+omega_deltaA) with HindIII and SacI (7 from 8 colonies confirmed). </p>
+Piotr<br>
+Checking if OmpA_omega_A_alpha gives ampicillin resistance
-Sprawdzanie czy omp_omega_A_alfa daje oporność na AP Piotr
+Inoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 into the same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100) in relation 1:50
-Zaszczepienie omp_omega_A_alfa z rużnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75; 1 do takich samych stężeń IPTG, ale z rużnymi stężeniami AP (25;50;75;100) w stosunku 1:50
+Bacterial growth (OD) measurement in the evening:
-wieczorem mieżenie OD
 <table id="result" align="center">
@@ Line 25: / Line 25: @@
-Sprawdzenie czy degradacja fuzji z Ompa jest wynikiem działania proteaz lon iompt (obecne w top10)
+Checking if degradation of fusion with OmpA is a result of the activity of lon and iompt proteases. (present in top10)
-PIOTR
-Transformacja omega_A_alfa i A_alfa do rosetta
+Transformation omega_A_alpha and A_alpha into Rosetta.