Team:Warsaw/Calendar-Main/30 July 2008
From 2008.igem.org
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<li>PCR to obtain truncated A protein DNA fragment <br> | <li>PCR to obtain truncated A protein DNA fragment <br> | ||
- | Primers: | + | Primers: |
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI ">AL+SacI</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI ">AL+SacI</a> | ||
- | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a><br> |
Elongation time: 30s <br> | Elongation time: 30s <br> |
Revision as of 22:52, 11 October 2008
Cloning of omega_A DNA fragment to pACYC177+OmpA_alphaMichał K.Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin. Cloning of truncated fragment of protein A DNAMichał K.
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