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Team:Warsaw/Calendar-Main/30 September 2008
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<h3>Preparation of BioBricks</h3> | <h3>Preparation of BioBricks</h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
| - | <ol | + | <ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of HQs(??????) plasmids.</li> |
| + | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of isolated plasmids with EcoRI and BcuI (BamHI buffer). </li> | ||
| + | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pACYC177+OmpA_omega_deltaA_alpha with BamHI and BglII (BamHI buffer). </li> | ||
| + | <li>Gel elctrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band: ??????. </li> </li> | ||
| + | |||
| + | <li>Gel elctrophoresis. Chioce of proper clone. </li> | ||
Revision as of 13:59, 24 October 2008
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Mutagenesis of protein APawełResults of sequencing: unfortunately all sequences where wild-type. Preparation of BioBricksMichał K. |
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