Team:Warsaw/Calendar-Main/31 July 2008

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<h4> Michał K.</h4>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with ligation products (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA). </li>
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<li> Transformants plating on LB + kanamycin. </li></ol></p>
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Revision as of 00:04, 12 October 2008

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Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day.
  2. Control digest of isolated plasmids with BamHI and SacI.
  3. Gel electrophoresis - proper colones founded.

Michał K.

  1. Transformation of E. coli TOP10 strain with ligation products (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA).
  2. Transformants plating on LB + kanamycin.




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