Team:Warsaw/Calendar-Main/31 July 2008

From 2008.igem.org

(Difference between revisions)
Line 5: Line 5:
<html>
<html>
-
<h3>Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha</h3><h4>Michał K.</h4>
+
<h3>Cloning of omega_A DNA fragment to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a></h3><h4>Michał K.</h4>
<p>
<p>

Revision as of 07:50, 16 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day.
  2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer).
  3. Gel electrophoresis - proper colones founded.

Cloning of truncated fragment of protein A

Michał K.

  1. Transformation of E. coli TOP10 strain with ligation products (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA).
  2. Transformants plating on LB + kanamycin.




Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/31_July_2008"