Team:Warsaw/Calendar-Main/31 July 2008
From 2008.igem.org
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<img src="https://static.igem.org/mediawiki/2008/e/e7/July_31_st.jpg" width=350 /><var>Fig. 1. <b>Pellets from Z-omega induction with various IPTG concentrations<br> | <img src="https://static.igem.org/mediawiki/2008/e/e7/July_31_st.jpg" width=350 /><var>Fig. 1. <b>Pellets from Z-omega induction with various IPTG concentrations<br> | ||
The arrow shows place of our overexpressed protein:</b><br> | The arrow shows place of our overexpressed protein:</b><br> | ||
- | 1. 22°C 0 | + | 1. 22°C 0.5 mM IPTG<br> |
- | 2. 22°C 0 | + | 2. 22°C 0.1 mM IPTG<br> |
3. 22°C 1 mM IPTG<br> | 3. 22°C 1 mM IPTG<br> | ||
4. Marker<br> | 4. Marker<br> | ||
5. negative control<br> | 5. negative control<br> | ||
- | 6. 37°C 0 | + | 6. 37°C 0.1 mM IPTG<br> |
- | 7. 37°C 0 | + | 7. 37°C 0.5 mM IPTG<br> |
8. 37°C 1 mM IPTG<br></var> | 8. 37°C 1 mM IPTG<br></var> | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2008/6/68/July_31_st_bis.jpg" width=350 /><var>Fig. 2. <b>Pellets and supernatants from Z-alpha induction with various IPTG concentrations<br> | ||
+ | The arrow shows place of our overexpressed protein:</b><br> | ||
+ | 1. Marker<br> | ||
+ | 2. pellet negative control<br> | ||
+ | 3. supernatant negative contro<br> | ||
+ | 4. pellet 37°C 0.1 mM IPTG <br> | ||
+ | 5. supernatant 37°C 0.1 mM IPTG<br> | ||
+ | 6. sonicate 22°C 0.1 mM IPTG<br> | ||
+ | 7. sonicate 22°C 0.5 mM IPTG<br> | ||
+ | 8. pellet 37°C 0.5 mM IPTG<br> | ||
+ | 9. supernatant 37°C 0.5 mM IPTG<br></var> | ||
</html> | </html> | ||
Revision as of 16:09, 24 October 2008
Purification of proteins: Z-alpha and Z-omegaPiotr, Emilia
Cloning of omega_A DNA fragment to pACYC177+OmpA_alphaMichał K.
Cloning of truncated fragment of protein AMichał K.The arrow shows place of our overexpressed protein: 1. 22°C 0.5 mM IPTG 2. 22°C 0.1 mM IPTG 3. 22°C 1 mM IPTG 4. Marker 5. negative control 6. 37°C 0.1 mM IPTG 7. 37°C 0.5 mM IPTG 8. 37°C 1 mM IPTG Fig. 2. Pellets and supernatants from Z-alpha induction with various IPTG concentrations The arrow shows place of our overexpressed protein: 1. Marker 2. pellet negative control 3. supernatant negative contro 4. pellet 37°C 0.1 mM IPTG 5. supernatant 37°C 0.1 mM IPTG 6. sonicate 22°C 0.1 mM IPTG 7. sonicate 22°C 0.5 mM IPTG 8. pellet 37°C 0.5 mM IPTG 9. supernatant 37°C 0.5 mM IPTG
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