Team:Warsaw/Calendar-Main/3 June 2008
From 2008.igem.org
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E.coli</i> TOP10 with ligation product.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E.coli</i> TOP10 with ligation product.</li> | ||
<li>Plating transformants on LB+tetracycline.</li> | <li>Plating transformants on LB+tetracycline.</li> | ||
- | <li> | + | <li>Digest of pMPM-T5 + transcription fusion with EcoRHI and HindIII.</li> |
<li>Use of T4 polymerase for blunting.</li> | <li>Use of T4 polymerase for blunting.</li> | ||
<li>DNA gel electrophoresis.</li> | <li>DNA gel electrophoresis.</li> |
Revision as of 17:54, 1 October 2008
Preparation of T7 constructs
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