Team:Warsaw/Calendar-Main/3 June 2008
From 2008.igem.org
(Difference between revisions)
E.szczesna (Talk | contribs) |
|||
Line 10: | Line 10: | ||
<li>Digest of DNA with HindIII and SalI (2x Tango buffer).</li> | <li>Digest of DNA with HindIII and SalI (2x Tango buffer).</li> | ||
- | <li>Digest of pMPM-T5 + AID with HindIII and SalI.</li> | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5 + AID with HindIII and SalI.</li> |
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up of reaction products.</a></li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up of reaction products.</a></li> | ||
<li>Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).</li> | <li>Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).</li> |
Revision as of 18:03, 1 October 2008
Preparation of T7 constructs
|