Team:Warsaw/Calendar-Main/3 June 2008

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Revision as of 18:04, 5 October 2008


	Attribution analyzer / Interactive list of topics

DNA (PCR products from previous day) gel electrophoresis and isolation of DNA from proper band (3300 bp).

Digest of pMPMT5-AID+T7 (transcription fusion) with EcoRHI and HindIII (2x Tango buffer).
Use of T4 polymerase for blunting.
DNA gel electrophoresis.
Isolation of DNA from proper band (7000 bp).
Ligation of product p.11 (pMPM-T5 + T7 RNA-polymerase).
Transformation of E.coli TOP10 with ligation product.
Plating transformants on LB + tetracycline.

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 <ol>
-<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPMT5 + transcription fusion with EcoRHI and HindIII (2x Tango buffer).</li>
+<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPMT5-AID+T7 (transcription fusion) with EcoRHI and HindIII (2x Tango buffer).</li>
 <li>Use of T4 polymerase for <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting"> blunting</a>.</li>
 <li>DNA gel electrophoresis.</li>
-<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA</a> from proper band.</li>
+<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA</a> from proper band (7000 bp).</li>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of product p.11 (pMPM-T5 + T7 RNA-polymerase).</li>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation product.</li>