Team:Warsaw/Calendar-Main/3 June 2008

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<var>Fig. 1. PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</var>
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<var><b>Fig. 1.</b> PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</var>
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Revision as of 15:30, 11 October 2008

Gallery Bricks Notebook Team Project Home


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Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

DNA (PCR products from previous day) gel electrophoresis and isolation of DNA from proper band (20 cycles - 3300 bp). Fig. 1.

Preparation of pMPMT5+T7 construct

Piotr

  1. Digest of pMPMT5-AID+T7 (transcription fusion) with EcoRHI and HindIII (2x Tango buffer).
  2. Use of T4 polymerase for blunting (3 hr of incubation).
  3. DNA gel electrophoresis.
  4. Isolation of DNA from proper band (7000 bp).
  5. Ligation of isolated DNA (pMPM-T5 + T7 RNA-polymerase).
  6. Transformation of E.coli TOP10 with ligation product.
  7. Plating transformants on LB + tetracycline.


Fig. 1. PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.

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