Team:Warsaw/Calendar-Main/3 June 2008
From 2008.igem.org
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- | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7 </a>(transcriptional fusion) with | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7 </a>(transcriptional fusion) with EcoRI and HindIII (2x Tango buffer).</li> |
<li>Use of T4 polymerase for <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting"> blunting</a> (3 hr of incubation).</li> | <li>Use of T4 polymerase for <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting"> blunting</a> (3 hr of incubation).</li> | ||
<li>DNA gel electrophoresis.</li> | <li>DNA gel electrophoresis.</li> |
Revision as of 16:53, 12 October 2008
Preparation of pMPMT5-AID+AIDT7 constructMichał K.DNA (PCR products from previous day) gel electrophoresis and isolation of DNA from proper band (20 cycles - 3300 bp). Fig. 1. Preparation of pMPMT5+T7 constructPiotr
Fig. 1. PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct. Lane 1 - 25 cycles, lane 2 - 20 cycles. |