Team:Warsaw/Calendar-Main/4 August 2008

From 2008.igem.org

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<p>Inoculation of  some pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA colonies of tranformnts.</p>
<p>Inoculation of  some pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA colonies of tranformnts.</p>
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<h3>Checking the expression of omp_omega_A_alpha and omp_A_alpha</h3><h4>Piotr</h4>
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<h3>Checking the expression of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>omp_omega_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>omp_A_alpha</a></h3><h4>Piotr</h4>
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<p>Inoculation of omp_omega_A_alpha and omp_A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.</p>
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<p>Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>omp_omega_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>omp_A_alpha</a> with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.</p>
<h3>Checking if omp_omega_A_alpha gives ampicillin resistance</h3><h4>Piotr</h4>
<h3>Checking if omp_omega_A_alpha gives ampicillin resistance</h3><h4>Piotr</h4>
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<p>Inoculation of omp_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL.</p>
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<p>Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>omp_omega_A_alpha</a> into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL.</p>
<h3>Preparing pACYC177+OmpA_omega_deltaA construct</h3><h4>Michał K.</h4>
<h3>Preparing pACYC177+OmpA_omega_deltaA construct</h3><h4>Michał K.</h4>

Revision as of 07:53, 16 October 2008

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Cloning of truncated fragment of protein A

Piotr

Inoculation of some pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA colonies of tranformnts.

Checking the expression of omp_omega_A_alpha and omp_A_alpha

Piotr

Inoculation of omp_omega_A_alpha and omp_A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.

Checking if omp_omega_A_alpha gives ampicillin resistance

Piotr

Inoculation of omp_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL.

Preparing pACYC177+OmpA_omega_deltaA construct

Michał K.

  1. Digest of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI (BamHI buffer). DNA ends blunting blunting with Klenow fragment (3 hr).
  2. Gel elctrophoresis and gel-out of proper band - 4300 bp.
  3. Overnight ligation of isolated DNA fragment.