Team:Warsaw/Calendar-Main/5 August 2008
From 2008.igem.org
(Difference between revisions)
Line 12: | Line 12: | ||
- | <h3>Checking if OmpA_omega_A_alpha gives ampicillin resistance</h3> | + | <h3>Checking if <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_A_alpha</a> gives ampicillin resistance</h3> |
<h4>Piotr</h4> | <h4>Piotr</h4> | ||
- | <p>Inoculation OmpA_omega_A_alpha from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50.</p> | + | <p>Inoculation <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_A_alpha</a> from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50.</p> |
<center><h4 style="text-align: center">Measurement of bacterial culture growth (OD) in the evening:</h4> </center> | <center><h4 style="text-align: center">Measurement of bacterial culture growth (OD) in the evening:</h4> </center> | ||
Line 30: | Line 30: | ||
</table> | </table> | ||
<p> | <p> | ||
- | Inoculation of OmpA_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (replication is necessary)</p> | + | Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_A_alpha</a> into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (replication is necessary)</p> |
- | <h3>Checking OmpA_omega_A_alpha and OmpA_A_alpha expression</h3> | + | <h3>Checking <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>OmpA_A_alpha</a> expression</h3> |
<h4>Piotr</h4> | <h4>Piotr</h4> | ||
Revision as of 07:55, 16 October 2008
Cloning of truncated fragment of protein APiotr
Checking if OmpA_omega_A_alpha gives ampicillin resistancePiotrInoculation OmpA_omega_A_alpha from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50. Measurement of bacterial culture growth (OD) in the evening:
Inoculation of OmpA_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (replication is necessary) Checking OmpA_omega_A_alpha and OmpA_A_alpha expressionPiotr
Preparing pACYC177+OmpA_omega_deltaA constructMichał K.
|