Team:Warsaw/Calendar-Main/5 August 2008

From 2008.igem.org

(Difference between revisions)
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Sprawdzenie czy ekspresja A jest na powieżchni
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<h3>Checking for presence of A on the cell membrane<br>
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Piotr
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Nakroplenie bakterii na nittrocelulozę, wysuszenie, blokowanie, przeciwciała anty-A, płukanie, przeciwciała anty-królik, wywołanie BCIP i NBT
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Piotr</h3>
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<p><ol>
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<li>Pouring bacteria by drops onto nitrocellulose.</li>
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<li>Drying.</li>
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<li>Blocking.</li>
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<li>Anti-A antibody binding.</li>
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<li>Washing.</li>
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<li>Anti-rabbit antibody binding.</li>
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<li>Developing with BCIP and NBT.</li>
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</ol>
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</p>
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zdjęcie żelu
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[photo of the gel is to be placed here]

Revision as of 21:49, 8 October 2008

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  1. Isolation of plasmids from cultures inocluated on previous day.
  2. Control digest of isolated plasmids with BamHI and SacI.

Checking for presence of A on the cell membrane
Piotr

  1. Pouring bacteria by drops onto nitrocellulose.
  2. Drying.
  3. Blocking.
  4. Anti-A antibody binding.
  5. Washing.
  6. Anti-rabbit antibody binding.
  7. Developing with BCIP and NBT.

[photo of the gel is to be placed here]


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