Team:Warsaw/Calendar-Main/5 August 2008

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Cloning of truncated fragment of protein A

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day.
  2. Control diegest with SacI and BamHI (BamHI buffer).
  3. Gel electrophoresis.

Checking for presence of A on the cell membrane
Piotr

  1. Pouring bacteria by drops onto nitrocellulose
  2. Drying
  3. Blocking
  4. Anti-A antibody binding
  5. Washing
  6. Anti-rabbit antibody binding
  7. Developing with BCIP and NBT

[photo of the gel is to be placed here]


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