Team:Warsaw/Calendar-Main/5 May 2008

From 2008.igem.org

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<p>Piotr: <br>
<p>Piotr: <br>
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Setup of overnight culture <i>E. coli</i> TOP10 (LB broth) and Rosetta (LB broth + chloramphenicol) for preparation of chemocompetent bacteria and isolation of genomic DNA.<br>
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Setup of overnight culture <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> (LB broth) and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> (LB broth + chloramphenicol) for preparation of chemocompetent bacteria and isolation of genomic DNA.<br>
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<br>
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'''TOP10 (Invitrogen)'''<br>
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<br>
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F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- <br>
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<br>
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'''Rosetta(DE3)pLysS'''<br>
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<br>
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F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)<br>  
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</p>
</p>

Revision as of 15:42, 1 October 2008

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Piotr:
Setup of overnight culture E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> (LB broth) and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> (LB broth + chloramphenicol) for preparation of chemocompetent bacteria and isolation of genomic DNA.

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