Team:Warsaw/Calendar-Main/6 May 2008

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<div style="photo">Probes of isolated genomic DNA after mechanical destruction. 1 and 3-<i>E.coli</i> Rosetta DNA; 2-DNA ladder; 4-<i>E.coli</i> TOP10 DNA.</div>
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<em>Probes of isolated genomic DNA after mechanical destruction. 1 and 3-<i>E.coli</i> Rosetta DNA; 2-DNA ladder; 4-<i>E.coli</i> TOP10 DNA.</em>
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Revision as of 21:59, 8 October 2008

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Piotr:

1. Preparation of electro- and chemocompetent bacteria E. coli TOP10.
2. Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320) on LB broth with proper antibiotics.

Michał L.:

1. Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from E. coli TOP10 strain was used as a negative control.

Probes of isolated genomic DNA after mechanical destruction. 1 and 3-E.coli Rosetta DNA; 2-DNA ladder; 4-E.coli TOP10 DNA.