Team:Warsaw/Calendar-Main/8 September 2008
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- | <li> | + | <li>Supernatant containing A-alpha was mixed with NiNta bead in proportion 10:1 and placed on rotating platform for 30 min at 4°C</li> |
<li>Bead was spun down at 6000 RPM, 5 min at 4°C</li> | <li>Bead was spun down at 6000 RPM, 5 min at 4°C</li> | ||
<li>Supernatant was discarded and equal amount of Elution buffer 1 (Sonication buffer A suplemented with 50 mM imidazole) was placed on top of the bead</li> | <li>Supernatant was discarded and equal amount of Elution buffer 1 (Sonication buffer A suplemented with 50 mM imidazole) was placed on top of the bead</li> |
Revision as of 17:56, 24 October 2008