Team:Waterloo/Notebook
From 2008.igem.org
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==Protocols== | ==Protocols== | ||
- | [[Team:Waterloo/Notebook/Miniprep|1-2-3- Mini prep]] | + | * [[Team:Waterloo/Notebook/Miniprep|1-2-3- Mini prep]] |
+ | * [[Team:Waterloo/Notebook/Colony PCR|Colony PCR]] | ||
+ | * [[Team:Waterloo/Notebook/Diagnostic Digestion|Diagnostic Digestion]] | ||
+ | * [[Team:Waterloo/Notebook/DNA Digestion|DNA Digestion]] | ||
+ | * [[Team:Waterloo/Notebook/DNA Extraction|DNA Extraction]] | ||
+ | * [[Team:Waterloo/Notebook/Gel Electrophoresis|Gel Electrophoresis]] | ||
+ | * [[Team:Waterloo/Notebook/Ligation|Ligation]] | ||
+ | * [[Team:Waterloo/Notebook/Glycerol Stock Preparation|Glycerol Stock Preparation]] | ||
+ | * [[Team:Waterloo/Notebook/Inoculation|Inoculation]] | ||
+ | * [[Team:Waterloo/Notebook/Transformation|Transformation]] | ||
==Recipes== | ==Recipes== |
Revision as of 15:50, 24 October 2008
Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Sponsors |
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Contents |
Notebook
This year we are using a Google Notebook to co-ordinate our lab activities among team members. This includes our day to day activities and our progress.
Tentative Plans
Notes:
' denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene
" denotes that the construct contians the ribosome binding site, coding region for that gene, and a transcriptional terminator (TT)
Construction Stages
1) Construction of CI"
2) Construction of Plac CI"
3) Ligate Plac (reverse) TT and Plac CI"
CI Repression Test
1) Construct Pconst CI?
2) Construct PR CI rfp"
3) Ligate above two constructs together (Pconst CI" PR CI rfp")
4) Test above repression by CI
Synthesize
1) Plac (reverse) TT
2) TT (reverse) PR
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Protocols
- 1-2-3- Mini prep
- Colony PCR
- Diagnostic Digestion
- DNA Digestion
- DNA Extraction
- Gel Electrophoresis
- Ligation
- Glycerol Stock Preparation
- Inoculation
- Transformation