Team:Waterloo/Notebook
From 2008.igem.org
(→Protocols) |
|||
Line 53: | Line 53: | ||
==Protocols== | ==Protocols== | ||
+ | The list of protocols used can be found on the [[Team:Waterloo/Sponsors/Protocols|Protocols]] page. | ||
* [[Team:Waterloo/Notebook/Miniprep|1-2-3- Mini-prep]] | * [[Team:Waterloo/Notebook/Miniprep|1-2-3- Mini-prep]] | ||
* [[Team:Waterloo/Notebook/Colony PCR|Colony PCR]] | * [[Team:Waterloo/Notebook/Colony PCR|Colony PCR]] | ||
Line 62: | Line 63: | ||
* [[Team:Waterloo/Notebook/Inoculation|Inoculation]] | * [[Team:Waterloo/Notebook/Inoculation|Inoculation]] | ||
* [[Team:Waterloo/Notebook/Transformation|Transformation]] | * [[Team:Waterloo/Notebook/Transformation|Transformation]] | ||
+ | * [[Team:Waterloo/Notebook/Competent Cells|Making Competent Cells]] | ||
==Recipes== | ==Recipes== | ||
* [[Team:Waterloo/Notebook/Making AI Media|Making Auto Induction Media]] | * [[Team:Waterloo/Notebook/Making AI Media|Making Auto Induction Media]] | ||
* [[Team:Waterloo/Notebook/Making LB Media|Making LB Media]] | * [[Team:Waterloo/Notebook/Making LB Media|Making LB Media]] | ||
- | |||
* [[Team:Waterloo/Notebook/Making SOB Media|Making SOB Media]] | * [[Team:Waterloo/Notebook/Making SOB Media|Making SOB Media]] |
Revision as of 20:56, 28 October 2008
Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Sponsors |
---|
Contents |
Notebook
This year we are using a Google Notebook to co-ordinate our lab activities among team members. This includes our day to day activities and our progress.
Tentative Plans
Notes:
' denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene
" denotes that the construct contians the ribosome binding site, coding region for that gene, and a transcriptional terminator (TT)
Construction Stages
1) Construction of CI"
2) Construction of Plac CI"
3) Ligate Plac (reverse) TT and Plac CI"
CI Repression Test
1) Construct Pconst CI?
2) Construct PR CI rfp"
3) Ligate above two constructs together (Pconst CI" PR CI rfp")
4) Test above repression by CI
Synthesize
1) Plac (reverse) TT
2) TT (reverse) PR
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|
Protocols
The list of protocols used can be found on the Protocols page.
- 1-2-3- Mini-prep
- Colony PCR
- DNA Digestion
- DNA Extraction
- Gel Electrophoresis
- Ligation
- Glycerol Stock Preparation
- Inoculation
- Transformation
- Making Competent Cells