Team:Waterloo/Notebook

From 2008.igem.org

(Difference between revisions)
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==Protocols==
==Protocols==
The list of protocols used can be found on the [[Team:Waterloo/Notebook/Protocols|Protocols]] page.
The list of protocols used can be found on the [[Team:Waterloo/Notebook/Protocols|Protocols]] page.
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* [[Team:Waterloo/Notebook/Miniprep|1-2-3- Mini-prep]]
 
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* [[Team:Waterloo/Notebook/Colony PCR|Colony PCR]]
 
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* [[Team:Waterloo/Notebook/DNA Digestion|DNA Digestion]]
 
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* [[Team:Waterloo/Notebook/DNA Extraction|DNA Extraction]]
 
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* [[Team:Waterloo/Notebook/Gel Electrophoresis|Gel Electrophoresis]]
 
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* [[Team:Waterloo/Notebook/Ligation|Ligation]]
 
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* [[Team:Waterloo/Notebook/Glycerol Stock Preparation|Glycerol Stock Preparation]]
 
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* [[Team:Waterloo/Notebook/Inoculation|Inoculation]]
 
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* [[Team:Waterloo/Notebook/Transformation|Transformation]]
 
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* [[Team:Waterloo/Notebook/Competent Cells|Making Competent Cells]]
 
==Recipes==
==Recipes==
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* [[Team:Waterloo/Notebook/Making AI Media|Making Auto Induction Media]]
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Our recipes for making media can be found on the [[Team:Waterloo/Notebook/Recipes|Recipes]] page.
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* [[Team:Waterloo/Notebook/Making LB Media|Making LB Media]]
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* [[Team:Waterloo/Notebook/Making SOB Media|Making SOB Media]]
+

Revision as of 21:13, 28 October 2008

Home The Team The Project Parts Submitted to the Registry Modeling Notebook Sponsors

Contents

Notebook

This year we are using a Google Notebook to co-ordinate our lab activities among team members. This includes our day to day activities and our progress.

Tentative Plans

Notes:
' denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene
" denotes that the construct contians the ribosome binding site, coding region for that gene, and a transcriptional terminator (TT)

Construction Stages
1) Construction of CI"
2) Construction of Plac CI"
3) Ligate Plac (reverse) TT and Plac CI"

CI Repression Test
1) Construct Pconst CI?
2) Construct PR CI rfp"
3) Ligate above two constructs together (Pconst CI" PR CI rfp")
4) Test above repression by CI

Synthesize
1) Plac (reverse) TT
2) TT (reverse) PR

Lab Calendar

May
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
June
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
July
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31
August
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
October
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
November
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
December
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

Protocols

The list of protocols used can be found on the Protocols page.

Recipes

Our recipes for making media can be found on the Recipes page.