Team:Waterloo/Notebook/Making LB Media

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Making LB Media

Materials
500 mL media bottle or rack of test tubes
1 L beaker
Magnetic stir bar
Tryptone
Yeast extract
Sodium chloride
Granulate agar (only for solid media)

Instructions
1. Fill 1 L beaker with 500mL of deionized water.
2. Add magetic stir bar into beaker and on to magnetic stirrer. Turn to medium speed.
3. Measure and add slowly with a scupula:
- 5g tryptone
- 2.5g yeast extract
- 2.5 g sodium chloride
4. Once dissolved in the deionized water, pour in media bottle or aliquot 5mL in tubes.
5. If making solid media, add 7.5g granulated agar to the media bottle. DO NOT add it to the beaker.
6. Autoclave slightly open bottle/tubes on slow exchange.