Template:Team:CityColSanFrancisco/Notebook/Notebook/CCSF Protocols

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(New page: <Br> BASIC TRANSFORMATION PROTOCOL FOR USING CCSF COMPETENT CELLS 1. Thaw competent cells on ice for 10 min. 2. Pipet 200uL cells into chilled 1.5mL rube containing 1 uL plasmid of interes...)
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BASIC TRANSFORMATION PROTOCOL FOR USING CCSF COMPETENT CELLS
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BASIC TRANSFORMATION PROTOCOL FOR USING CCSF COMPETENT CELLS<br>
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1. Thaw competent cells on ice for 10 min.
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1. Thaw competent cells on ice for 10 min.<br>
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2. Pipet 200uL cells into chilled 1.5mL rube containing 1 uL plasmid of interest
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2. Pipet 200uL cells into chilled 1.5mL rube containing 1 uL plasmid of interest <br>
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3. Mix gently by tapping
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3. Mix gently by tapping <br>
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4. Incubate cells on ice in presence of plasmid for ~20 min.
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4. Incubate cells on ice in presence of plasmid for ~20 min. <br>
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5. Heat shock at 42C for 90 sec.
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5. Heat shock at 42C for 90 sec. <br>
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6. Return to ice for 2 min.
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6. Return to ice for 2 min. <br>
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7. Plate 100uL of transformation onto LB-amp plate
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7. Plate 100uL of transformation onto LB-amp plate <br>

Revision as of 19:23, 9 July 2009


BASIC TRANSFORMATION PROTOCOL FOR USING CCSF COMPETENT CELLS
1. Thaw competent cells on ice for 10 min.
2. Pipet 200uL cells into chilled 1.5mL rube containing 1 uL plasmid of interest
3. Mix gently by tapping
4. Incubate cells on ice in presence of plasmid for ~20 min.
5. Heat shock at 42C for 90 sec.
6. Return to ice for 2 min.
7. Plate 100uL of transformation onto LB-amp plate