Template:Team:CityColSanFrancisco/Notebook/Notebook/CCSF Protocols

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BASIC TRANSFORMATION PROTOCOL FOR USING CCSF COMPETENT CELLS 1. Thaw competent cells on ice for 10 min. 2. Pipet 200uL cells into chilled 1.5mL rube containing 1 uL plasmid of interest 3. Mix gently by tapping 4. Incubate cells on ice in presence of plasmid for ~20 min. 5. Heat shock at 42C for 90 sec. 6. Return to ice for 2 min. 7. Plate 100uL of transformation onto LB-amp plate