Template:Team:CityColSanFrancisco/Notebook/Notebook/DV notes

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''Hello''
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Hello <br>
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6/29/2009
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R. palustris TIE-1 and S. oneidensis WT strains appear to have grown.  Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species is what we suspect.
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Plan now to:
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- inoculate liquid media with single colonies from these strains.
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-Inoculate
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- Re-streak individual colonies from these strains, along with TIE-3 (R. palustris) and MtrB and OmpB strains (S. oneidensis)
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Tomorrow:
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-Order designed primers
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-Grow mutants in liquid media<br>
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6/30/2009 <br>
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Grew mutants alongside WT Shewanella.  They don't look alike.  I'm suspicious.

Latest revision as of 17:37, 30 June 2009

Hello
6/29/2009 R. palustris TIE-1 and S. oneidensis WT strains appear to have grown. Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species is what we suspect. Plan now to: - inoculate liquid media with single colonies from these strains. -Inoculate - Re-streak individual colonies from these strains, along with TIE-3 (R. palustris) and MtrB and OmpB strains (S. oneidensis)

Tomorrow: -Order designed primers -Grow mutants in liquid media

6/30/2009
Grew mutants alongside WT Shewanella. They don't look alike. I'm suspicious.