Template:Team:CityColSanFrancisco/Notebook/Notebook/DV notes

From 2008.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 1: Line 1:
Hello <br>
Hello <br>
6/29/2009
6/29/2009
-
R. palustris TIE-1 and S. oneidensis WT strains appear to have grown.  Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species.
+
R. palustris TIE-1 and S. oneidensis WT strains appear to have grown.  Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species is what we suspect.
 +
Plan now to:
 +
- inoculate liquid media with single colonies from these strains.
 +
-Inoculate
 +
- Re-streak individual colonies from these strains, along with TIE-3 (R. palustris) and MtrB and OmpB strains (S. oneidensis)
 +
 
 +
Tomorrow:
 +
-Order designed primers
 +
-Grow mutants in liquid media<br>
 +
 
 +
6/30/2009 <br>
 +
Grew mutants alongside WT Shewanella.  They don't look alike.  I'm suspicious.

Latest revision as of 17:37, 30 June 2009

Hello
6/29/2009 R. palustris TIE-1 and S. oneidensis WT strains appear to have grown. Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species is what we suspect. Plan now to: - inoculate liquid media with single colonies from these strains. -Inoculate - Re-streak individual colonies from these strains, along with TIE-3 (R. palustris) and MtrB and OmpB strains (S. oneidensis)

Tomorrow: -Order designed primers -Grow mutants in liquid media

6/30/2009
Grew mutants alongside WT Shewanella. They don't look alike. I'm suspicious.