Template:Team:CityColSanFrancisco/Notebook/Notebook/DV notes

From 2008.igem.org

(Difference between revisions)
 
Line 9: Line 9:
Tomorrow:
Tomorrow:
-Order designed primers
-Order designed primers
-
-Grow mutants in liquid media
+
-Grow mutants in liquid media<br>
 +
 
 +
6/30/2009 <br>
 +
Grew mutants alongside WT Shewanella.  They don't look alike.  I'm suspicious.

Latest revision as of 17:37, 30 June 2009

Hello
6/29/2009 R. palustris TIE-1 and S. oneidensis WT strains appear to have grown. Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species is what we suspect. Plan now to: - inoculate liquid media with single colonies from these strains. -Inoculate - Re-streak individual colonies from these strains, along with TIE-3 (R. palustris) and MtrB and OmpB strains (S. oneidensis)

Tomorrow: -Order designed primers -Grow mutants in liquid media

6/30/2009
Grew mutants alongside WT Shewanella. They don't look alike. I'm suspicious.