Template:Team:UC Berkeley/Notebook/AL construction

From 2008.igem.org

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'''--[[User:Aronlau|Aronlau]] 00:35, 5 June 2008 (UTC)'''
 
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<pre>
 
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PCR al0001/al0002 on pSB3C6-Bjh1501    (508bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112300
 
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-----------------------------------------------
 
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al0001 Forward Biobricking of lambda lysozyme with start and stop codons
 
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CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
 
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al0002 Reverse Biobricking of lambda lysozyme with start and stop codons
 
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CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
 
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</pre>
 
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<pre>
 
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PCR al0003/al0002 on pSB3C6-Bjh1501    (505bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112301
 
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-----------------------------------------------
 
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al0003 Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
 
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CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
 
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al0002 Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
 
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CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
 
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</pre>
 
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<pre>
 
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PCR al0001/al0004 on pSB3C6-Bjh1501    (505bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112302
 
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-----------------------------------------------
 
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al0001 Forward Biobricking of lambda lysozyme with start codon and no start codon
 
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CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
 
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al0004 Reverse Biobricking of lambda lysozyme with start codon and no start codon
 
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CGttaGGATCCtacatcaatctctctgaccgttccgcc
 
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</pre>
 
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<pre>
 
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PCR al0003/al0004 on pSB3C6-Bjh1501    (502bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112303
 
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-----------------------------------------------
 
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al0003 Forward Biobricking of lambda lysozyme with no start/stop codon
 
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CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
 
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al0004 Reverse Biobricking of lambda lysozyme with no start/stop codon
 
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CGttaGGATCCtacatcaatctctctgaccgttccgc
 
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</pre>
 
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<pre>
 
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PCR al0005/al0002 on pSB3C6-Bjh1501    (512bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112304
 
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-----------------------------------------------
 
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al0005 Forward Biobricking of lambda lysozyme with spacer
 
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CCATAGAATTCatgAGATCTacatatggtagaaatcaataatcaacgtaaggcgttcctcg
 
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al0002 Reverse Biobricking of lambda lysozyme with spacer
 
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CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
 
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</pre>
 
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<pre>
 
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PCR al0006/al0002 on pSB3C6-Bjh1501    (527bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112305
 
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-----------------------------------------------
 
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al0006 Forward Biobricking of lambda lysozyme with rbs site
 
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CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
 
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al0002 Reverse Biobricking of lambda lysozyme with rbs site
 
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CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
 
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</pre>
 
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<pre>
 
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PCR al0007/al0008 on pSB3C6-Bjh1502    (349bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112306
 
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-----------------------------------------------
 
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al0007 Forward Biobricking of lambda holin with start and stop codons
 
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CCATAGAATTCatgAGATCTatgccagaaaaacatgacctg
 
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al0008 Reverse Biobricking of lambda holin with start and stop codons
 
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CGttaGGATCCttattgatttctaccatcttctactccggc
 
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</pre>
 
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<pre>
 
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PCR al0009/al0008 on pSB3C6-Bjh1502    (346bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112307
 
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-----------------------------------------------
 
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al0009 Forward Biobricking of lambda holin with stop codon and no start codon
 
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CCATAGAATTCatgAGATCTccagaaaaacatgacctg
 
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al0008 Reverse Biobricking of lambda holin with stop codon and no start codon
 
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CGttaGGATCCttattgatttctaccatcttctactccggc
 
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</pre>
 
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<pre>
 
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PCR al0007/al0010 on pSB3C6-Bjh1502    (346bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112308
 
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-----------------------------------------------
 
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al0007 Forward Biobricking of lambda holin with start and no stop codon
 
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CCATAGAATTCatgAGATCTatgccagaaaaacatgacctg
 
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al0010 Reverse Biobricking of lambda holin wtih start and no stop codon
 
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CGttaGGATCCttgatttctaccatcttctactccggc
 
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</pre>
 
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<pre>
 
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PCR al0009/al0010 on pSB3C6-Bjh1502    (343bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112309
 
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-----------------------------------------------
 
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al0009 Forward Biobricking of lambda holin with no start and stop codon
 
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CCATAGAATTCatgAGATCTccagaaaaacatgacctg
 
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al0010 Reverse Biobricking of lambda holin with no start and stop codon
 
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CGttaGGATCCttgatttctaccatcttctactccggc
 
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</pre>
 
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<pre>
 
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PCR al0011/al0008 on pSB3C6-Bjh1502    (353bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112310
 
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-----------------------------------------------
 
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al0011 Forward Biobricking of lambda holin with spacer
 
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CCATAGAATTCatgAGATCTacatatgccagaaaaacatgacctg
 
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al0008 Reverse Biobricking of lambda holin with spacer
 
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CGttaGGATCCttattgatttctaccatcttctactccggc
 
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</pre>
 
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<pre>
 
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PCR al0012/al0008 on pSB3C6-Bjh1502    (369bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112311
 
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-----------------------------------------------
 
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al0012 Forward Biobricking of lambda holin with rbs site
 
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???
 
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al0008 Reverse Biobricking of lambda holin with rbs site
 
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CGttaGGATCCttattgatttctaccatcttctactccggc
 
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</pre>
 
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<pre>
 
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PCR al0013/al0014 on pSB3C6-Bjh1503    (355bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112312
 
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-----------------------------------------------
 
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al0013 Forward Biobricking of lambda antiholin with start and stop codons
 
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CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
 
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al0014 Reverse Biobricking of lambda antiholin with start and stop codons
 
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CGttaGGATCCttattgatttctaccatcttctactccggc
 
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</pre>
 
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<pre>
 
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PCR al0015/al0014 on pSB3C6-Bjh1503    (352bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112313
 
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-----------------------------------------------
 
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al0015 Forward Biobricking of lambda antiholin with stop codon and no start codon
 
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CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
 
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al0014 Reverse Biobricking of lambda antiholin with stop codon and no start codon
 
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CGttaGGATCCttattgatttctaccatcttctactccggc
 
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</pre>
 
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<pre>
 
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PCR al0013/al0016 on pSB3C6-Bjh1503    (352bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112314
 
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-----------------------------------------------
 
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al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon
 
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CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
 
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al0016 Reverse Biobricking of lambda antiholin with start codon and no stop codon
 
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CGttaGGATCCttgatttctaccatcttctactccgg
 
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</pre>
 
-
 
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<pre>
 
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PCR al0015/al0016 on pSB3C6-Bjh1503    (349bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112315
 
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-----------------------------------------------
 
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al0015 Forward Biobricking of lambda antiholin with no start and stop codons
 
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CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
 
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al0016 Reverse Biobricking of lambda antiholin with no start and stop codons
 
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???
 
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</pre>
 
-
 
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<pre>
 
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PCR al0017/al0014 on pSB3C6-Bjh1503    (359bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112316
 
-
-----------------------------------------------
 
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al0017 Forward Biobricking of lambda antiholin with spacer
 
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al0014 Reverse Biobricking of lambda antiholin with spacer
 
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CGttaGGATCCttattgatttctaccatcttctactccggc
 
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</pre>
 
-
 
-
<pre>
 
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PCR al0018/al0014 on pSB3C6-Bjh1503    (375bp, EcoRI/BamHI/DpnI)
 
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Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
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Product is pBca1256-K112317
 
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-----------------------------------------------
 
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al0018 Forward Biobricking of lambda antiholin with rbs site
 
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CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc
 
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al0014 Reverse Biobricking of lambda antiholin with rbs site
 
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CGttaGGATCCttattgatttctaccatcttctactccggc
 
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</pre>
 

Revision as of 00:43, 5 June 2008