"
Template:Team:UC Berkeley/Notebook/AL construction
From 2008.igem.org
(Difference between revisions)
| Line 133: | Line 133: | ||
<pre> | <pre> | ||
| - | PCR al0013/ | + | PCR al0013/al0008 on pSB3C6-Bjh1503 (355bp, EcoRI/BamHI/DpnI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112312 | Product is pBca1256-K112312 | ||
| Line 139: | Line 139: | ||
al0013 Forward Biobricking of lambda antiholin with start and stop codons | al0013 Forward Biobricking of lambda antiholin with start and stop codons | ||
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc | CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc | ||
| - | + | al0008 Reverse Biobricking of lambda antiholin with start and stop codons | |
CGttaGGATCCttattgatttctaccatcttctactccggc | CGttaGGATCCttattgatttctaccatcttctactccggc | ||
</pre> | </pre> | ||
<pre> | <pre> | ||
| - | PCR | + | PCR al0014/al0008 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112313 | Product is pBca1256-K112313 | ||
----------------------------------------------- | ----------------------------------------------- | ||
| - | + | al0014 Forward Biobricking of lambda antiholin with stop codon and no start codon | |
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg | CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg | ||
| - | + | al0008 Reverse Biobricking of lambda antiholin with stop codon and no start codon | |
CGttaGGATCCttattgatttctaccatcttctactccggc | CGttaGGATCCttattgatttctaccatcttctactccggc | ||
</pre> | </pre> | ||
<pre> | <pre> | ||
| - | PCR al0013/ | + | PCR al0013/al0010 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112314 | Product is pBca1256-K112314 | ||
| Line 161: | Line 161: | ||
al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon | al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon | ||
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc | CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc | ||
| - | + | al0010 Reverse Biobricking of lambda antiholin with start codon and no stop codon | |
| - | + | CGttaGGATCCttgatttctaccatcttctactccggc | |
</pre> | </pre> | ||
<pre> | <pre> | ||
| - | PCR | + | PCR al0014/al0010 on pSB3C6-Bjh1503 (349bp, EcoRI/BamHI/DpnI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112315 | Product is pBca1256-K112315 | ||
----------------------------------------------- | ----------------------------------------------- | ||
| - | + | al0014 Forward Biobricking of lambda antiholin with no start and stop codons | |
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg | CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg | ||
| - | + | al0010 Reverse Biobricking of lambda antiholin with no start and stop codons | |
| - | + | CGttaGGATCCttgatttctaccatcttctactccggc | |
</pre> | </pre> | ||
<pre> | <pre> | ||
| - | PCR | + | PCR al0015/al0008 on pSB3C6-Bjh1503 (359bp, EcoRI/BamHI/DpnI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112316 | Product is pBca1256-K112316 | ||
----------------------------------------------- | ----------------------------------------------- | ||
| - | + | al0015 Forward Biobricking of lambda antiholin with spacer | |
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc | CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc | ||
| - | + | al0008 Reverse Biobricking of lambda antiholin with spacer | |
CGttaGGATCCttattgatttctaccatcttctactccggc | CGttaGGATCCttattgatttctaccatcttctactccggc | ||
</pre> | </pre> | ||
<pre> | <pre> | ||
| - | PCR | + | PCR al0016/al0008 on pSB3C6-Bjh1503 (375bp, EcoRI/BamHI/DpnI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112317 | Product is pBca1256-K112317 | ||
----------------------------------------------- | ----------------------------------------------- | ||
| - | + | al0016 Forward Biobricking of lambda antiholin with rbs site | |
CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc | CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc | ||
| - | + | al0008 Reverse Biobricking of lambda antiholin with rbs site | |
CGttaGGATCCttattgatttctaccatcttctactccggc | CGttaGGATCCttattgatttctaccatcttctactccggc | ||
</pre> | </pre> | ||
Revision as of 03:14, 5 June 2008
--Aronlau 01:15, 5 June 2008 (UTC)
PCR al0001/al0002 on pSB3C6-Bjh1501 (508bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112300 ----------------------------------------------- al0001 Forward Biobricking of lambda lysozyme with start and stop codons CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg al0002 Reverse Biobricking of lambda lysozyme with start and stop codons CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
PCR al0003/al0002 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112301 ----------------------------------------------- al0003 Forward Biobricking of Lambda Lysozyme with stop codon and no start codon CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg al0002 Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
PCR al0001/al0004 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112302 ----------------------------------------------- al0001 Forward Biobricking of lambda lysozyme with start codon and no start codon CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg al0004 Reverse Biobricking of lambda lysozyme with start codon and no start codon CGttaGGATCCtacatcaatctctctgaccgttccgc
PCR al0003/al0004 on pSB3C6-Bjh1501 (502bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112303 ----------------------------------------------- al0003 Forward Biobricking of lambda lysozyme with no start/stop codon CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg al0004 Reverse Biobricking of lambda lysozyme with no start/stop codon CGttaGGATCCtacatcaatctctctgaccgttccgc
PCR al0005/al0002 on pSB3C6-Bjh1501 (512bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112304 ----------------------------------------------- al0005 Forward Biobricking of lambda lysozyme with spacer CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg al0002 Reverse Biobricking of lambda lysozyme with spacer CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
PCR al0006/al0002 on pSB3C6-Bjh1501 (527bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112305 ----------------------------------------------- al0006 Forward Biobricking of lambda lysozyme with rbs site CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg al0002 Reverse Biobricking of lambda lysozyme with rbs site CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
PCR al0007/al0008 on pSB3C6-Bjh1502 (349bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112306 ----------------------------------------------- al0007 Forward Biobricking of lambda holin with start and stop codons CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg al0008 Reverse Biobricking of lambda holin with start and stop codons CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0009/al0008 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112307 ----------------------------------------------- al0009 Forward Biobricking of lambda holin with stop codon and no start codon CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc al0008 Reverse Biobricking of lambda holin with stop codon and no start codon CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0007/al0010 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112308 ----------------------------------------------- al0007 Forward Biobricking of lambda holin with start and no stop codon CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg al0010 Reverse Biobricking of lambda holin wtih start and no stop codon CGttaGGATCCttgatttctaccatcttctactccggc
PCR al0009/al0010 on pSB3C6-Bjh1502 (343bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112309 ----------------------------------------------- al0009 Forward Biobricking of lambda holin with no start and stop codon CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc al0010 Reverse Biobricking of lambda holin with no start and stop codon CGttaGGATCCttgatttctaccatcttctactccggc
PCR al0011/al0008 on pSB3C6-Bjh1502 (353bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112310 ----------------------------------------------- al0011 Forward Biobricking of lambda holin with spacer CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg al0008 Reverse Biobricking of lambda holin with spacer CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0012/al0008 on pSB3C6-Bjh1502 (369bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112311 ----------------------------------------------- al0012 Forward Biobricking of lambda holin with rbs site CCATAGAATTCatgAGATCTattgggggtaagacCtgaagatgccag al0008 Reverse Biobricking of lambda holin with rbs site CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0013/al0008 on pSB3C6-Bjh1503 (355bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112312 ----------------------------------------------- al0013 Forward Biobricking of lambda antiholin with start and stop codons CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc al0008 Reverse Biobricking of lambda antiholin with start and stop codons CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0014/al0008 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112313 ----------------------------------------------- al0014 Forward Biobricking of lambda antiholin with stop codon and no start codon CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg al0008 Reverse Biobricking of lambda antiholin with stop codon and no start codon CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0013/al0010 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112314 ----------------------------------------------- al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc al0010 Reverse Biobricking of lambda antiholin with start codon and no stop codon CGttaGGATCCttgatttctaccatcttctactccggc
PCR al0014/al0010 on pSB3C6-Bjh1503 (349bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112315 ----------------------------------------------- al0014 Forward Biobricking of lambda antiholin with no start and stop codons CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg al0010 Reverse Biobricking of lambda antiholin with no start and stop codons CGttaGGATCCttgatttctaccatcttctactccggc
PCR al0015/al0008 on pSB3C6-Bjh1503 (359bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112316 ----------------------------------------------- al0015 Forward Biobricking of lambda antiholin with spacer CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc al0008 Reverse Biobricking of lambda antiholin with spacer CGttaGGATCCttattgatttctaccatcttctactccggc
PCR al0016/al0008 on pSB3C6-Bjh1503 (375bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112317 ----------------------------------------------- al0016 Forward Biobricking of lambda antiholin with rbs site CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc al0008 Reverse Biobricking of lambda antiholin with rbs site CGttaGGATCCttattgatttctaccatcttctactccggc
