Template:Team:UC Berkeley/Notebook/BX construction

From 2008.igem.org

(Difference between revisions)
(Sixpi 22:25, 4 June 2008 (UTC))
Line 42: Line 42:
bx4 Reverse Biobricking of {<T4 Holin>}
bx4 Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
cgttaGGATCCtttagcccttcctaatattctggcc
 +
</pre>
 +
 +
<pre>
 +
PCR bx3/bx4 on Bjh1301         (692 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI)
 +
Product is pBca1256-K112004
 +
----------------------------------------
 +
bx5 Forward Biobricking of {a~T4 Holin!}
 +
CCATAGAATTCatgAGATCTgtctatggcagcacctagaat
 +
bx2 Reverse Biobricking of {a~T4 Holin!}
 +
cgttaGGATCCttatttagcccttcctaatattct
 +
</pre>
 +
 +
<pre>
 +
PCR bx6/bx2 on Bjh1301 (701 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256 (EcoRI/BamHI)
 +
Product is pBca1256-K112005
 +
----------------------------------------
 +
bx6 Forward Biobricking of {rbs.T4 Holin!}
 +
CCATAGAATTCatgAGATCTgaagaaaaaacatatggcagcacctagaat
 +
bx2 Reverse Biobricking of {rbs.T4 Holin!}
 +
cgttaGGATCCttatttagcccttcctaatattct
 +
</pre>
 +
 +
<pre>
 +
</pre>
</pre>

Revision as of 22:49, 4 June 2008

Sixpi 22:25, 4 June 2008 (UTC)

PCR bx1/bx2 on Bjh1301		        (688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1		Forward Biobricking of {T4 Holin!}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx2		Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx3/bx2 on Bjh1301		        (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3		Forward biobricking of {<T4 Holin!}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx2		Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattc
PCR bx1/bx4 on Bjh1301		        (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1		Forward biobricking of {T4 Holin>}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx4		Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
PCR bx3/bx4 on Bjh1301		        (682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3		Forward Biobricking of {<T4 Holin>}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx4		Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
PCR bx3/bx4 on Bjh1301		        (692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5		Forward Biobricking of {a~T4 Holin!}
CCATAGAATTCatgAGATCTgtctatggcagcacctagaat
bx2		Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx6/bx2 on Bjh1301		(701 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6		Forward Biobricking of {rbs.T4 Holin!}
CCATAGAATTCatgAGATCTgaagaaaaaacatatggcagcacctagaat
bx2		Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct


Bing Xia