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Template:Team:UC Berkeley/Notebook/BX construction
From 2008.igem.org
(Difference between revisions)
(→Sixpi 22:25, 4 June 2008 (UTC)) |
(→Sixpi 22:25, 4 June 2008 (UTC)) |
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==[[User:Sixpi|Sixpi]] 22:25, 4 June 2008 (UTC)== | ==[[User:Sixpi|Sixpi]] 22:25, 4 June 2008 (UTC)== | ||
<pre> | <pre> | ||
| - | PCR bx1/bx2 on Bjh1301 | + | PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI) |
Product is pBca1256-K112000 | Product is pBca1256-K112000 | ||
---------------------------------------- | ---------------------------------------- | ||
| Line 12: | Line 12: | ||
<pre> | <pre> | ||
| - | PCR bx3/bx2 on Bjh1301 | + | PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI) |
Product is pBca1256-K112001 | Product is pBca1256-K112001 | ||
---------------------------------------- | ---------------------------------------- | ||
| Line 23: | Line 23: | ||
<pre> | <pre> | ||
| - | PCR bx1/bx4 on Bjh1301 | + | PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI) |
Product is pBca1256-K112002 | Product is pBca1256-K112002 | ||
---------------------------------------- | ---------------------------------------- | ||
| Line 34: | Line 34: | ||
<pre> | <pre> | ||
| - | PCR bx3/bx4 on Bjh1301 | + | PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI) |
Product is pBca1256-K112003 | Product is pBca1256-K112003 | ||
---------------------------------------- | ---------------------------------------- | ||
| Line 45: | Line 45: | ||
<pre> | <pre> | ||
| - | PCR bx3/bx4 on Bjh1301 | + | PCR bx3/bx4 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI) |
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI) |
Product is pBca1256-K112004 | Product is pBca1256-K112004 | ||
---------------------------------------- | ---------------------------------------- | ||
| Line 57: | Line 57: | ||
<pre> | <pre> | ||
PCR bx6/bx2 on Bjh1301 (701 bp, EcoRI/BamHI/DpnI) | PCR bx6/bx2 on Bjh1301 (701 bp, EcoRI/BamHI/DpnI) | ||
| - | Sub into pBca1256 | + | Sub into pBca1256 (EcoRI/BamHI) |
Product is pBca1256-K112005 | Product is pBca1256-K112005 | ||
---------------------------------------- | ---------------------------------------- | ||
| Line 67: | Line 67: | ||
<pre> | <pre> | ||
| + | PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112006 | ||
| + | ---------------------------------------- | ||
| + | bx7 Forward biobricking of {T4 Antiholin!} | ||
| + | CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac | ||
| + | bx8 Reverse biobricking of {T4 Antiholin!} | ||
| + | cgttaGGATCCtcattcagtctccaatttaatg | ||
| + | </pre> | ||
| + | <pre> | ||
| + | PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112007 | ||
| + | ---------------------------------------- | ||
| + | bx9 Forward biobricking of {<T4 Antiholin!} | ||
| + | CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt | ||
| + | bx8 Reverse biobricking of {<T4 Antiholin!} | ||
| + | cgttaGGATCCtcattcagtctccaatttaatg | ||
</pre> | </pre> | ||
| + | |||
| + | <pre> | ||
| + | PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112008 | ||
| + | ---------------------------------------- | ||
| + | bx7 Forward biobricking of {T4 Antiholin>} | ||
| + | CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac | ||
| + | bx10 Reverse biobricking of {T4 Antiholin>} | ||
| + | cgttaGGATCCttcagtctccaatttaatgttc | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112009 | ||
| + | ---------------------------------------- | ||
| + | bx9 Forward biobricking of {<T4 Antiholin>} | ||
| + | CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt | ||
| + | bx10 Reverse biobricking of {<T4 Antiholin>} | ||
| + | cgttaGGATCCttcagtctccaatttaatgttc | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | PCR bx9/bx10 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112010 | ||
| + | ---------------------------------------- | ||
| + | bx11 Forward biobricking of {a~T4 Antiholin!} | ||
| + | CCATAGAATTCatgAGATCTctttatggccttaaaagcaacagc | ||
| + | bx8 Reverse biobricking of {a~T4 Antiholin!} | ||
| + | cgttaGGATCCtcattcagtctccaatttaatg | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | PCR bx12/bx8 on Bjh1303 (338 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112011 | ||
| + | ---------------------------------------- | ||
| + | bx12 Forward biobricking of {rbs.T4 Antiholin!} | ||
| + | CCATAGAATTCatgAGATCTgaagaaaaaacatatggccttaaaagcaacagc | ||
| + | bx8 Reverse biobricking of {rbs.T4 Antiholin!} | ||
| + | cgttaGGATCCtcattcagtctccaatttaatg | ||
| + | </pre> | ||
| + | |||
[[Team:UC_Berkeley/Notebook/Bing_Xia|Bing Xia]]<br> | [[Team:UC_Berkeley/Notebook/Bing_Xia|Bing Xia]]<br> | ||
Revision as of 23:16, 4 June 2008
Sixpi 22:25, 4 June 2008 (UTC)
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1 Forward Biobricking of {T4 Holin!}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx2 Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3 Forward biobricking of {<T4 Holin!}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx2 Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattc
PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1 Forward biobricking of {T4 Holin>}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx4 Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3 Forward Biobricking of {<T4 Holin>}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx4 Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
PCR bx3/bx4 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5 Forward Biobricking of {a~T4 Holin!}
CCATAGAATTCatgAGATCTgtctatggcagcacctagaat
bx2 Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx6/bx2 on Bjh1301 (701 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6 Forward Biobricking of {rbs.T4 Holin!}
CCATAGAATTCatgAGATCTgaagaaaaaacatatggcagcacctagaat
bx2 Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112006
----------------------------------------
bx7 Forward biobricking of {T4 Antiholin!}
CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8 Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112007
----------------------------------------
bx9 Forward biobricking of {<T4 Antiholin!}
CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt
bx8 Reverse biobricking of {<T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112008
----------------------------------------
bx7 Forward biobricking of {T4 Antiholin>}
CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac
bx10 Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc
PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112009
----------------------------------------
bx9 Forward biobricking of {<T4 Antiholin>}
CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt
bx10 Reverse biobricking of {<T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc
PCR bx9/bx10 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112010
----------------------------------------
bx11 Forward biobricking of {a~T4 Antiholin!}
CCATAGAATTCatgAGATCTctttatggccttaaaagcaacagc
bx8 Reverse biobricking of {a~T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
PCR bx12/bx8 on Bjh1303 (338 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112011
----------------------------------------
bx12 Forward biobricking of {rbs.T4 Antiholin!}
CCATAGAATTCatgAGATCTgaagaaaaaacatatggccttaaaagcaacagc
bx8 Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
